The GNL3L Antibody (PAC02367) is a crucial tool for researchers studying the GNL3L protein, which is involved in cell growth and proliferation. This polyclonal antibody, developed using rabbit immunization, exhibits high reactivity specifically with human samples and is optimized for use in Western blotting applications.GNL3L, also known as G Protein Nucleolar 3 Like, is a key player in cellular processes such as ribosome biogenesis and nucleolar organization. Dysregulation of GNL3L has been linked to cancer progression and other diseases, making it a promising target for therapeutic interventions.
With the ability to detect GNL3L protein in various cell types, this antibody is an essential tool for researchers in molecular biology, oncology, and cell biology. By understanding the role of GNL3L in cell function, researchers can uncover new insights into disease mechanisms and potentially develop novel treatment strategies.
Antibody Name:
GNL3L Antibody (PACO22367)
Antibody SKU:
PACO22367
Size:
100ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:500-1:3000
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from internal of human GNL3L.
Form:
Liquid
Storage Buffer:
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western blot analysis of extracts from HeLa cells, 293 cells and HT-29 cells, using GNL3L antibody.
Background:
Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP.
GNL3L: Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. Belongs to the MMR1/HSR1 GTP-binding protein family.Protein type: NucleolusChromosomal Location of Human Ortholog: Xp11.22Cellular Component: cytoplasm; membrane; mitochondrion; nucleolus; nucleus; telomerase holoenzyme complexMolecular Function: GTPase activity; protein bindingBiological Process: negative regulation of protein binding; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of telomere maintenance via telomerase; regulation of protein stability; ribosome biogenesis and assembly
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
