The GNL3L Polyclonal Antibody (PACO07093) is a valuable tool for researchers studying GNL3L, a protein involved in nucleolar processes and ribosome biogenesis. This antibody is raised in rabbits and exhibits high reactivity with human samples, making it ideal for use in Western blot applications. By binding to the GNL3L protein, this antibody enables the detection and analysis of GNL3L in various cell types, providing insights into its role in cellular functions.GNL3L is known for its involvement in ribosome assembly and its interaction with nucleolar proteins, indicating its importance in regulating protein synthesis and cell growth.
Research on GNL3L is particularly relevant in the fields of molecular biology and cancer research, as alterations in ribosome biogenesis can contribute to tumor development and progression. By studying GNL3L with the PACO07093 antibody, researchers can uncover the mechanisms underlying these processes and potentially identify new therapeutic targets for cancer treatment.
Antibody Name:
GNL3L Antibody (PACO07093)
Antibody SKU:
PACO07093
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, WB, IHC
Recommended Dilutions:
ELISA:1:5000, WB:1:500-1:2000, IHC:1:100-1:300
Species Reactivity:
Human
Immunogen:
Synthesized peptide derived from the Internal region of human GNL3L.
Form:
Liquid
Storage Buffer:
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Purification Method:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Western Blot analysis of NIH-3T3 LOVO cells using GNL3L Polyclonal Antibody.
Western blot analysis of mouse-brain HELA KB SH-SY5Y 293T 3T3 Mouse-kidney lysis using GNL3L antibody.
Western Blot analysis of HT29 cells using GNL3L Polyclonal Antibody.
Synonyms:
GNL3L; Guanine nucleotide-binding protein-like 3-like protein
UniProt Protein Function:
GNL3L: Stabilizes TERF1 telomeric association by preventing TERF1 recruitment by PML. Stabilizes TERF1 protein by preventing its ubiquitination and hence proteasomal degradation. Does so by interfering with TERF1-binding to FBXO4 E3 ubiquitin-protein ligase. Required for cell proliferation. By stabilizing TRF1 protein during mitosis, promotes metaphase-to-anaphase transition. Stabilizes MDM2 protein by preventing its ubiquitination, and hence proteasomal degradation. By acting on MDM2, may affect TP53 activity. Required for normal processing of ribosomal pre-rRNA. Binds GTP. Belongs to the MMR1/HSR1 GTP-binding protein family.Protein type: NucleolusChromosomal Location of Human Ortholog: Xp11.22Cellular Component: cytoplasm; membrane; mitochondrion; nucleolus; nucleus; telomerase holoenzyme complexMolecular Function: GTPase activity; protein bindingBiological Process: negative regulation of protein binding; negative regulation of protein sumoylation; negative regulation of protein ubiquitination; negative regulation of telomere maintenance via telomerase; regulation of protein stability; ribosome biogenesis and assembly
UniProt Protein Details:
NCBI Summary:
The protein encoded by this gene appears to be a nucleolar GTPase that is essential for ribosomal pre-rRNA processing and cell proliferation. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, May 2010]
