The GNL3 Polyclonal Antibody (CAB6459) is a valuable tool for researchers studying the GNL3 protein, also known as nucleostemin, which plays a crucial role in cell proliferation and stem cell maintenance. This antibody, raised in rabbits, offers high specificity and sensitivity for detecting GNL3 in human samples, making it ideal for use in Western blot applications.GNL3 is a nucleolar protein that has been implicated in various biological processes, including ribosome biogenesis, cell cycle regulation, and cancer development. Its overexpression has been observed in many types of cancer, suggesting a potential role in tumorigenesis.
By targeting GNL3 with this antibody, researchers can gain valuable insights into its function and potential as a therapeutic target for cancer treatment.Overall, the GNL3 Polyclonal Antibody is a versatile tool for researchers interested in investigating the role of GNL3 in cellular processes and disease development. Its high reactivity and validation for Western blotting make it a dependable choice for studies in cancer biology, stem cell research, and other fields related to nucleolar function.
Product Name:
GNL3 Rabbit Polyclonal Antibody
SKU:
CAB6459
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human GNL3 (NP_996562.1).
WB,1:500 - 1:2000 IF/ICC,1:10 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
NS; E2IG3; NNP47; C77032; GNL3
Positive Sample:
293T
Conjugate:
Unconjugated
Cellular Localization:
Nucleus, nucleolus.
Calculated MW:
62kDa
Observed MW:
62kDa
The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
26354
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from 293T cells, using GNL3 Rabbit pAb (CAB6459) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
