The GNAT3 Polyclonal Antibody (CAB15982) is specifically designed for research involving GNAT3, a protein responsible for mediating taste perception and signal transduction in taste receptor cells. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the GNAT3 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studies in taste perception and signal transduction research.
GNAT3, also known as gustducin alpha subunit 3, is a critical component in the taste signaling pathway, playing a key role in transmitting taste signals from taste receptors to the brain. Its involvement in taste perception makes it a target of interest in research on flavor preferences, taste disorders, and sensory perception. Understanding the function of GNAT3 is essential for unraveling the mechanisms underlying taste perception and developing strategies to manipulate taste sensation for therapeutic purposes.
Product Name:
GNAT3 Rabbit Polyclonal Antibody
SKU:
CAB15982
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 60-130 of human GNAT3 (NP_001095856.1).
Sequence:
GYSE QECM EFKA VIYS NTLQ SILA IVKA MTTL GIDY VNPR SAED QRQL YAMA NTLE DGGM TPQL AEVI KRL
Tested Applications:
WBIF/ICCELISA
Recommended Dilution:
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:200
Synonyms:
GDCA; HG1E; GNAT3
Positive Sample:
A-549
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm.
Calculated MW:
40kDa
Observed MW:
37kDa
Sweet, bitter, and umami tastes are transmitted from taste receptors by a specific guanine nucleotide binding protein. The protein encoded by this gene is the alpha subunit of this heterotrimeric G protein, which is found not only in the oral epithelium but also in gut tissues. Variations in this gene have been linked to metabolic syndrome.
Purification Method:
Affinity purification
Gene ID:
346562
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from A-549 cells, using GNAT3 Rabbit pAb (CAB15982) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
