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GNAS Rabbit Polyclonal Antibody (CAB2732)

SKU:
CAB2732
Product Type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
€139 - €419
Frequently bought together:

Description

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GNAS Rabbit Polyclonal Antibody (CAB2732)

The GNAS Polyclonal Antibody (CAB2732) is a vital tool for research involving the GNAS gene, which encodes the stimulatory G protein alpha subunit and plays a crucial role in numerous cellular signaling pathways. This antibody, produced in rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By binding to the GNAS protein, this antibody enables accurate detection and analysis in various cell types, making it an excellent choice for studies in molecular biology, endocrinology, and signal transduction research.

The GNAS gene is known for its involvement in several genetic disorders, including Albright hereditary osteodystrophy and McCune-Albright syndrome, which are characterized by skeletal abnormalities and hormonal imbalances. Additionally, mutations in GNAS have been linked to various hormone-related cancers, such as pituitary adenomas and ovarian tumors. Understanding the function and regulation of the GNAS gene is essential for unraveling the molecular mechanisms underlying these diseases and developing targeted therapies to combat them.

Product Name:GNAS Rabbit Polyclonal Antibody
SKU:CAB2732
Size:20uL, 100uL
Isotype:IgG
Host Species:Rabbit
Reactivity:Human,Mouse
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GNAS (P63092).
Sequence:MGCL GNSK TEDQ RNEE KAQR EANK KIEK QLQK DKQV YRAT HRLL LLGA GESG KSTI VKQM RILH VNGF NGEG GEED PQAA RSNS DGEK ATKV QDIK NNLK
Tested Applications:WB ELISA
Recommended Dilution:WB,1:500 - 1:2000
Synonyms:AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45; GNAS
Positive Sample:NCI-H460,SW620,Mouse skeletal muscle,Mouse brain,Mouse heart
Conjugate:Unconjugated
Cellular Localization:Cell membrane By similarity; Lipid-anchor By similarity.
Calculated MW:28kDa/44kDa/45kDa/77kDa/109kDa/111kDa
Observed MW:43kDa

This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.

Purification Method:Affinity purification
Gene ID:2778
Storage Buffer:Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of extracts of various cell lines, using GNAS antibody (CAB2732) at 1:400 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

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