The GMDS Polyclonal Antibody (CAB15060) is a valuable tool for researchers studying GMDS, a key enzyme involved in nucleotide sugar metabolism. This antibody, raised in rabbits, is highly specific for human samples and has been validated for use in various applications, including Western blotting. By binding to the GMDS protein, this antibody enables the detection and analysis of GMDS in a variety of cell types, making it an ideal choice for studies in biochemistry and molecular biology.GMDS plays a crucial role in the biosynthesis of GDP-fucose, a sugar molecule that serves as a key component of glycoproteins and glycolipids.
Disruptions in GMDS function have been linked to various diseases, including congenital disorders and cancer. By studying GMDS expression and activity, researchers can gain valuable insights into the role of nucleotide sugar metabolism in health and disease. This antibody provides a powerful tool for investigating the function of GMDS and its potential as a therapeutic target in a range of pathological conditions.
Product Name:
GMDS Rabbit Polyclonal Antibody
SKU:
CAB15060
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human GMDS (NP_001491.1).
GDP-mannose 4,6-dehydratase (GMD; EC 4.2.1.47) catalyzes the conversion of GDP-mannose to GDP-4-keto-6-deoxymannose, the first step in the synthesis of GDP-fucose from GDP-mannose, using NADP+ as a cofactor. The second and third steps of the pathway are catalyzed by a single enzyme, GDP-keto-6-deoxymannose 3,5-epimerase, 4-reductase, designated FX in humans (MIM 137020).
Purification Method:
Affinity purification
Gene ID:
2762
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from HeLa cells, using GMDS Rabbit pAb (CAB15060) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 60s.
