The Glypican-3 Polyclonal Antibody (CAB1946) is a valuable tool for researchers studying Glypican-3, a cell surface protein involved in various cellular processes, including cell growth, differentiation, and migration. This antibody, produced in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications.Glypican-3 is known for its role in promoting cell proliferation and tumor growth, making it a target of interest in cancer research. By specifically binding to Glypican-3, this antibody enables precise detection and analysis of the protein in different cell types, making it suitable for studies in cancer biology and developmental biology.
Understanding the function of Glypican-3 is crucial for developing targeted therapies for cancer and other diseases where dysregulation of this protein occurs. By using the Glypican-3 Polyclonal Antibody, researchers can further explore the role of Glypican-3 in cellular processes and its potential as a therapeutic target in oncology and regenerative medicine.
Product Name:
Glypican 3 (GPC3) Rabbit Polyclonal Antibody
SKU:
CAB1946
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 481-580 of human Glypican 3 (GPC3) (NP_004475.1).
Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2719
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Glypican 3 (GPC3) (GPC3) antibody (CAB1946) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
