Glycerol Colorimetric Assay Kit
- SKU:
- MAES0225
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Detection Method:
- Colorimetric
- Sample Type:
- Serum, Plasma, Animal Tissue, Cell
- Sensitivity:
- 0.01mmol/L
- Range:
- 0.01-1.00 mmol/L
- Assay Time:
- 10 min
Frequently bought together:
Description
Product Name: | Glycerol Colorimetric Assay Kit |
Product Code: | MAES0225 |
Sample Type: | Serum (Plasma), Animal Tissue, Cell |
Assay Time: | 10 min |
Assay Type: | Quantitative |
Detection Instrument: | Microplate reader (500-520 nm, optimum wavelength: 510 nm) |
Detection Method: | Colorimetric method |
Sensitivity: | 0.01mmol/L |
Detection Range: | 0.01-1.00 mmol/L |
Inter-assay CV: | 0.08 |
Intra-assay CV: | 0.04 |
Glycerol is transformed by enzyme to produce hydrogen peroxide, which is catalyzed by oxidase in the presence of 4-amino-antipyrprid and phenol to produce red quinones, the color of which is proportional to the content of glycerol.
Storage: | 2-8°C |
Valid Period: | 12 months |
Other Reagents Required: | Double distilled water, Isopropanol (AR), PBS(0.01 M,pH 7.4) |
Other Instruments Required: | Water bath |