Glycerol Assay Kit (BA0115)

SKU:

BA0115

Product Type:

Assay

Instrument:

Microplate Reader

Sample Type:

Biological, Food, Beverage

Research Area:

Food Safety & Analysis

Plant & Environmental Stress

Clinical Chemistry

Description

Description

Glycerol Assay Kit

Glycerol [Glycerin or Glycerine, C₃H₅ (OH) ₃] is widely used in foods, beverages and pharmaceutical formulations. It is also a main by-product of biodiesel production. Simple, direct and automation-ready procedures for measuring glycerol concentrations find wide applications.

Assay Genie's glycerol assay uses a single Working Reagent that combines glycerol kinase, glycerol phosphate oxidase and color reactions in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at Ex/Em = 530/585nm is directly proportional to glycerol concentration in the sample.

Applications

For quantitative determination of glycerol and evaluation of drug effects on glycerol metabolism.

Glycerol Assay Kit -Key Features

Sensitive and accurate. Use as little as 10 uL samples. Linear detection range in 96-well plate: 10 to 1000 uM (92 ug/dL to 9.2 mg/dL) glycerol for colorimetric assays and 2 to 50 for fluorimetric assays.
Simple and convenient. The procedure involves addition of a single working reagent and incubation for 20 min at room temperature, compatible for HTS assays.
Improved reagent stability. The optimized formulation has greatly enhanced the reagent and signal stability.

Glycerol Assay Kit Information:

Kit Includes:	Assay Buffer: 24 mL Enzyme Mix: 500 mL ATP: 250 mL Dye Reagent: 220 mL Standard: 100 mL 100 mM Glycerol
Kit Requires:	Pipeting devices, centrifuge tubes, Clear flat-bottom 96-well plates, black 96-well plates and plate reader.
Method of Detection:	OD570nm, or FL530/585nm
Detection Limit:	2 uM
Samples:	Biological, food, beverage etc
Species:	All
Protocol Length:	20 min
Size:	200 tests
Storage:	Store all components at -20°C
Shelf Life:	12 months