Glutathione Peroxidase (GSH-Px) Activity Assay Kit (MAES0084)
- SKU:
- MAES0084
- Product Type:
- Assay
- Instrument:
- Microplate Reader
- Sample Type:
- Serum, Plasma, Cells, Cell Culture Supernatant, Tissue
- Sensitivity:
- 17.17 U
- Range:
- 17.17-518.32 U
- Assay Time:
- 40 min
- Shipping:
- Gel Pack
- Research Area:
- Oxidative Stress
- Antioxidant
- Diabetes & Obesity
- Plant & Environmental Stress
- Enzyme Activity
Description
| Product Name: | Glutathione Peroxidase Activity Assay Kit |
| Product Code: | MAES0084 |
| Product Size: | 96 Assays |
| Sample Type: | Serum, plasma, cells, cell culture supernatant, tissue |
| Assay Time: | 40 min |
| Instrument: | Microplate reader |
| Sensitivity: | 17.17 U |
| Detection Range: | 17.17-518.32 U |
| Recovery Rate: | 104 |
| Inter CV: | 6.2 |
| Inter CV: | 2.4 |
Glutathione peroxidase (GSH-Px) is an important enzyme that catalyzes decomposition of hydrogen peroxide. GSH specifically catalyze the reaction between GSH and hydrogen peroxide, protecting cell membrane structure and keeping membrane function integrity. Se-cysteine is the active center of the GSH-Px. Determination of GSH-Px activity in organism can be an indicator of selenium level as Se is essential section of GSH-Px.
Glutathione peroxidase (GSH-Px) can promote the reaction of hydrogen peroxide (H2O2) and reduced glutathione to produce H2O and oxidized glutathione (GSSG). The activity of glutathione peroxidase can be expressed by the rate of enzymatic reaction. The activity of glutathione can be calculated by measuring the consumption of reduced glutathione. Hydrogen peroxide (H2O2) and reduced glutathione can react without catalysis of GSH- Px, so the portion of GSH reduction by non-enzymatic reaction should be subtracted. GSH can react with dinitrobenzoic acid to produce 5-thio-dinitrobenzoic acid anion, which showed a stable yellow color. Measure the absorbance at 412nm, and calculate the amount of GSH.
| Shelf Life: | 6 Months |
| Storage: | 2-8°C |
| Shipping: | Gel Pack |
| Research Area: | Enzymes, Oxidative stress |
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