Glutaryl-HIST1H2BC (K116) Antibody (PACO60520)
- SKU:
- PACO60520
- Product Type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Applications:
- ELISA
- WB
- IF
- Antibody Type:
- Polyclonal Antibody
- Conjugation:
- Unconjugated
Description
Glutaryl-HIST1H2BC (K116) Antibody (PACO60520)
The Glutaryl-Hist1H2BC K116 Polyclonal Antibody (PACO60520) is a valuable tool for researchers studying histone proteins and their post-translational modifications. This antibody, generated in rabbits, specifically targets the K116 residue of the Glutaryl-Hist1H2BC protein, allowing for precise detection and analysis in various experimental settings.Histone proteins, including Hist1H2BC, play a crucial role in chromatin structure and gene regulation. Post-translational modifications, such as glutarylation, can impact the function of histones and ultimately influence gene expression. The Glutaryl-Hist1H2BC K116 Polyclonal Antibody is validated for use in Western blot applications, providing researchers with a reliable tool for investigating the role of glutarylation in chromatin dynamics and gene regulation.
By targeting the K116 residue of Glutaryl-Hist1H2BC, this antibody enables researchers to study specific post-translational modifications and their functional implications. Its high reactivity with human samples makes it suitable for a wide range of research applications in fields such as epigenetics, cancer biology, and developmental biology. Gain valuable insights into the complex mechanisms of gene regulation with the Glutaryl-Hist1H2BC K116 Polyclonal Antibody.
| Antibody Name: | Glutaryl-HIST1H2BC (K116) Antibody (PACO60520) |
| Antibody SKU: | PACO60520 |
| Size: | 50ul |
| Host Species: | Rabbit |
| Tested Applications: | ELISA, WB, IF |
| Recommended Dilutions: | ELISA:1:2000-1:10000, WB:1:100-1:1000, IF:1:1-1:10 |
| Species Reactivity: | Human |
| Immunogen: | Peptide sequence around site of Glutaryl-Lys (116) derived from Human Histone H2B type 1-C/E/F/G/I |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
| Purification Method: | Antigen Affinity Purified |
| Clonality: | Polyclonal |
| Isotype: | IgG |
| Conjugate: | Non-conjugated |
 | Western Blot. Positive WB detected in: 293 whole cell lysate (treated with 30mM sodium butyrate for 4h). All lanes: HIST1H2BC antibody at 1.27µg/ml. Secondary. Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 14 kDa. Observed band size: 14 kDa. |
 | Immunofluorescence staining of Hela cells (treated with 30mM sodium butyrate for 4h) with PACO60520 at 1:5, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). |
| Background: | Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| Synonyms: | Histone H2B type 1-C/E/F/G/I (Histone H2B.1 A) (Histone H2B.a) (H2B/a) (Histone H2B.g) (H2B/g) (Histone H2B.h) (H2B/h) (Histone H2B.k) (H2B/k) (Histone H2B.l) (H2B/l), HIST1H2BC; HIST1H2BE; HIST1H2BF; HIST1H2BG; HIST1H2BI, H2BFL; H2BFH; H2BFG; H2BFA; H2BFK |
| UniProt Protein Function: | H2B1C: a core component of the nucleoosome. The nucleosome, a basic organizational unit of chromosomal DNA, is octrameric, consisting of two molecules each of histones H2B, H2A, H3, H4. The octamer wraps approximately 147 bp of DNA. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. |
| UniProt Protein Details: | Protein type:DNA-binding Chromosomal Location of Human Ortholog: 6p22.1 Cellular Component: cytoplasm; extracellular space; nucleoplasm; nucleus Molecular Function:DNA binding; protein binding Biological Process: antibacterial humoral response; defense response to Gram-positive bacterium; innate immune response in mucosa; nucleosome assembly |
| NCBI Summary: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. The protein has antibacterial and antifungal antimicrobial activity. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| UniProt Code: | P62807 |
| NCBI GenInfo Identifier: | 290457686 |
| NCBI Gene ID: | 8339 |
| NCBI Accession: | P62807.4 |
| UniProt Secondary Accession: | P62807,P02278, Q3B872, Q4VB69, Q93078, Q93080, |
| UniProt Related Accession: | P62807 |
| Molecular Weight: | 13,906 Da |
| NCBI Full Name: | Histone H2B type 1-C/E/F/G/I |
| NCBI Synonym Full Names: | histone cluster 1, H2bg |
| NCBI Official Symbol: | HIST1H2BGÂ Â |
| NCBI Official Synonym Symbols: | H2B/a; H2BFA; H2B.1A; dJ221C16.8Â Â |
| NCBI Protein Information: | histone H2B type 1-C/E/F/G/I |
| UniProt Protein Name: | Histone H2B type 1-C/E/F/G/I |
| UniProt Synonym Protein Names: | Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l |
| Protein Family: | Histone |
| UniProt Gene Name: | HIST1H2BCÂ Â |
| UniProt Entry Name: | H2B1C_HUMAN |
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