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Glutamine Colorimetric Assay Kit

SKU:
MAES0235
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Tissue, Cells And Cell Culture Supernatan
Sensitivity:
0.036 mmol/L
Range:
0.036-2.0 mmol/L
Assay Time:
70 min
€509
Frequently bought together:

Description

Product Name:Glutamine Colorimetric Assay Kit
Product Code:MAES0235
Sample Type:Serum (Plasma), Tissue, Cells And Cell Culture Supernatan
Assay Time:70 min
Assay Type:Quantitative
Synonyms:Gln
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Detection Method:Colorimetric method
Sensitivity:0.036 mmol/L
Detection Range:0.036–2.0 mmol/L
Inter-assay CV:0.047
Intra-assay CV:0.032

Gln is hydrolyzed to produce glutamic acid under the action of glutaminase. Glutamic acid is further catalyzed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The content of Gln can be calculated by measuring the change of absorbance value at 450 nm.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:Normal saline (0.9% NaCl)
Other Instruments Required:Incubator, 50 KD Ultrafiltration tube

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