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Glutamine Assay Kit (Colorimetric) (BA0113)

SKU:
BA0113
Product Type:
Assay
Detection Method:
Colorimetric
Instrument:
Microplate Reader
Sample Type:
Serum, Plasma, Animal Tissue, Red Blood Cells, Cultured Cells
Research Area:
Ferroptosis
Plant & Environmental Stress
Amino Acid and Protein
Clinical Chemistry
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$1,247
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Description

ELISA Kit Technical ManualMSDS

Glutamine Assay Kit - Information

Glutamine is an amino acid synthesized in the muscle that plays major roles in protein synthesis, acid-base balance, anabolic processes and is utilized for cellular energy and as a carbon source. It is used in treatment of injury, trauma, burns, and also as a supplement for muscle growth and post-surgery healing. Simple, direct and automation-ready procedures for measuring glutamine concentration are very desirable.

Assay Genie's glutamine assay kit is based on hydrolysis of glutamine to glutamate and colorimetric determination of the product. The intensity of the product color, measured at 565 nm, is proportional to the glutamine concentration in the sample.

Applications

For quantitative determination of glutamine and evaluation of drug effects on glutamine metabolism.

Key Features

  • Sensitive and accurate. Use 20 uL sample. Linear detection range 0.023 - 2 mM glutamine in 96-well plate assay.
  • Convenient. The procedure involves adding a single working reagent, incubation for 40 min at room temperature, adding a stop reagent and reading the optical density. No 37°C heater is needed.
  • High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.

Glutamine Assay Kit Information

Kit Includes: Assay Buffer: 15 mL NAD Solution: 1 mL Enzyme A: 120 mL MTT Solution: 2 x 1.5 mL Enzyme B: 220 mL Stop Reagent: 25 mL Standard: 400 mL 100 mM Glutamine
Kit Requires: Pipeting (multi-channel) devices. Clear flat-bottom 96-well plates and plate reader.
Method of Detection: OD565nm
Detection Limit: 23 uM
Samples: Serum, plasma, urine, cell, tissue etc
Species: All
Protocol Length: 40 min
Size: 100 tests
Storage: Store all reagents at -20°C
Shelf Life: 6 months

 

Neto et al.2P-FLIM unveils time-dependent metabolic shifts during osteogenic differentiation with a key role of lactate to fuel osteogenesis via glutaminolysis identifiedbioRxiv 2023View Citation

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