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Glutaminase (GLS) Activity Assay Kit

SKU:
MAES0230
Product Type:
Assay
Instrument:
Microplate Reader
Detection Method:
Colorimetric
Sample Type:
Serum, Plasma, Animal, Plant Tissue
Sensitivity:
0.003 U/L
Range:
0.003-18.0 U/L
Assay Time:
80min
€389
Frequently bought together:

Description

Product Name:Glutaminase (GLS) Activity Assay Kit
Product Code:MAES0230
Sample Type:Serum (Plasma), Animal, Plant Tissue
Assay Time:80min
Assay Type:Enzyme Activity
Synonyms:GLS
Detection Instrument:Microplate reader (440-460 nm, optimum wavelength: 450 nm)
Detection Method:Colorimetric method
Sensitivity:0.003 U/L
Detection Range:0.003-18.0 U/L
Inter-assay CV:0.083
Intra-assay CV:0.05

Glutamine is decomposed to produce glutamic acid under the action of glutaminase. Glutamic acid is further transformed by glutamic acid dehydrogenase. Meanwhile, NAD+ is reduced to NADH, Which under the action of hydrogen transmitter, transfer electrons to WST-8 to produce the yellow product. The activity of glutaminase can be calculated by measuring the change of absorbance value at 450 nm.

Storage:-20°C
Valid Period:12 months
Other Reagents Required:PBS (0.01 mol/L, pH 7.4)
Other Instruments Required:Test tube, Incubator, Centrifuge

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