The Glucosylceramidase Polyclonal Antibody (CAB8420) is a powerful tool for research related to glucosylceramidase, an enzyme involved in the metabolism of ceramide and glycosphingolipids. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards human samples, making it suitable for use in Western blot applications.Glucosylceramidase plays a crucial role in lipid metabolism and lysosomal function, making it a key player in various biological processes such as cell growth, differentiation, and apoptosis. Dysregulation of this enzyme has been linked to lysosomal storage disorders and metabolic diseases, underscoring its importance in human health.
By targeting glucosylceramidase, researchers can gain insights into its function and potential implications in disease pathology. This antibody enables the detection and analysis of glucosylceramidase in different cell types, offering new opportunities for studying its role in metabolic disorders, neurodegenerative diseases, and cancer. Its versatility and reliability make it an essential tool for advancing research in these fields.
Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human Glucosylceramidase beta (Glucosylceramidase beta (GBA)) (NP_000148.2).
This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2629
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of MCF-7 cells, using Glucosylceramidase beta (Glucosylceramidase beta (GBA)) antibody (CAB8420) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
