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Glucose-6-Phosphate Assay Kit (BA0111)

SKU:
BA0111
Product Type:
Assay
Instrument:
Microplate Reader
Sample Type:
Plasma, Serum, Tissue, Culture Media
Research Area:
Glycolysis & Carbohydrates
Pentose Phosphate
Oxidative Stress
Antioxidant
Diabetes & Obesity
Plant & Environmental Stress
Clinical Chemistry
€939
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Description

ELISA Kit Technical ManualMSDS

Glucose-6-Phosphate Assay Kit

Glucose-6-Phosphate (g6p) is glucose sugar phosphorylated on carbon 6. Most of the glucose entering cells is phosphorylated to G6P. G6P has three primary fates within the cell. It lies at the start of two major metabolic pathways: glycolysis and the pentose phosphate pathway. In addition to these metabolic pathways, glucose 6-phosphate may also be converted to glycogen or starch for storage.

Assay Genie's Glucose-6-Phosphate Assay Kit provides a simple, and automation-ready procedure for measuring G6P concentration. G6P is oxidized by glucose-6-phosphate dehydrogenase and the formed NADPH is coupled to the formazan (WST-8) chromogen. The intensity of the product color, measured at 460 nm, is proportional to the G6P concentration in the sample.

Applications

For quantitative determination of glucose-6-phosphate in biological samples.

Key Features

  • Fast and sensitive. Linear detection range: 10 to 1000 uM G6P.
  • Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Glucose-6-Phosphate Assay Kit Information:

Kit Includes: Assay Buffer: 10 mL Enzyme A: 120 uL NADP/WST8: 1 mL Enzyme B: 120 uL Standard (100 mM G6P): 100 uL
Kit Requires: Pipetting devices and accessories (e.g. multi-channel pipettor), clear flat-bottom 96-well centrifuge tubes and plate reader
Method of Detection: OD460nm
Detection Limit: 10 uM
Samples: Plasma, serum, tissue and culture media etc
Species: All
Protocol Length: 20 min
Size: 100 tests
Storage: Store all kit components at -20 °C.
Shelf Life: 6 months

 

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