The Glucokinase Polyclonal Antibody (CAB6293) is a vital tool for researchers studying the enzyme glucokinase, which plays a crucial role in glucose metabolism and pancreatic beta cell function. This antibody, produced in rabbits, is highly specific to human samples and has been validated for use in Western blotting and immunohistochemistry applications. By binding to glucokinase, this antibody allows for accurate detection and analysis of the enzyme in various tissues and cell types.Glucokinase is a key enzyme involved in regulating blood glucose levels by phosphorylating glucose in the liver and pancreatic beta cells.
Dysregulation of glucokinase activity is associated with several metabolic disorders, including diabetes and obesity. Research into the function and regulation of glucokinase is essential for developing new therapeutic strategies for these conditions.Overall, the Glucokinase Polyclonal Antibody (CAB6293) is a valuable tool for scientists interested in exploring the role of glucokinase in metabolic diseases and diabetes research. Its high specificity and reliability make it an essential component of any lab conducting studies in this field.
Product Name:
Glucokinase (GCK) Rabbit Polyclonal Antibody
SKU:
CAB6293
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Glucokinase (GCK) (NP_000153.1).
This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia.
Purification Method:
Affinity purification
Gene ID:
2645
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using Glucokinase (GCK) Rabbit pAb (CAB6293) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 60s.
