The GLS2 Polyclonal Antibody (CAB16029) is a key tool for researchers studying GLS2, a crucial enzyme involved in glutamine metabolism. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been specifically validated for use in Western blot applications.GLS2, also known as glutaminase 2, plays a vital role in the conversion of glutamine to glutamate, a process essential for cellular energy production and metabolism. Dysregulation of GLS2 has been implicated in various diseases, including cancer and metabolic disorders. By targeting GLS2 with this antibody, researchers can gain valuable insights into the role of glutamine metabolism in disease pathogenesis.
With its ability to specifically bind to GLS2 protein, this antibody enables researchers to detect and analyze GLS2 expression in a variety of cell types, making it an invaluable tool for studies in cancer biology, metabolism, and metabolic disorders. Understanding the function of GLS2 at the molecular level is critical for developing targeted therapies that exploit the glutamine metabolism pathway in the treatment of various diseases.
Product Name:
GLS2 Rabbit Polyclonal Antibody
SKU:
CAB16029
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 545-602 of human GLS2 (NP_037399.2).
The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.
Purification Method:
Affinity purification
Gene ID:
27165
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GLS2 antibody (CAB16029) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
