The GLS Polyclonal Antibody (CAB3885) is a valuable tool for researchers studying GLS, a critical enzyme involved in glutaminolysis and cancer metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity towards human GLS samples, making it an ideal choice for Western blot applications. By targeting the GLS protein, researchers can effectively detect and analyze its expression in various cell types, aiding in the investigation of metabolic pathways in cancer and other diseases.
GLS, also known as glutaminase, is essential for the conversion of glutamine to glutamate, a crucial step in cell metabolism and proliferation. Dysregulation of GLS activity has been linked to various cancers and metabolic disorders, making it a promising target for therapeutic interventions. By studying the function of GLS using this antibody, researchers can gain valuable insights into the metabolic reprogramming of cancer cells and potentially identify novel treatment strategies.
Product Name:
Glutaminase (GLS) Rabbit Polyclonal Antibody
SKU:
CAB3885
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 610-669 of human Glutaminase (Glutaminase (GLS)) (NP_055720.3).
This gene encodes the K-type mitochondrial glutaminase. The encoded protein is an phosphate-activated amidohydrolase that catalyzes the hydrolysis of glutamine to glutamate and ammonia. This protein is primarily expressed in the brain and kidney plays an essential role in generating energy for metabolism, synthesizing the brain neurotransmitter glutamate and maintaining acid-base balance in the kidney. Alternate splicing results in multiple transcript variants.
Purification Method:
Affinity purification
Gene ID:
2744
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using Glutaminase (Glutaminase (GLS)) antibody (CAB3885) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
