The GLIS3 Polyclonal Antibody (CAB12166) is a valuable tool for researchers studying GLIS3, a transcription factor known for its role in controlling gene expression and cellular processes. This antibody, derived from rabbits, exhibits high specificity towards human samples and has been validated for use in Western blot applications. By binding specifically to the GLIS3 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for investigations in molecular biology and developmental research.GLIS3 is involved in the regulation of various biological functions, including pancreatic beta cell development, insulin secretion, and kidney development.
Dysregulation of GLIS3 has been associated with a number of diseases, such as diabetes, congenital hypothyroidism, and nephronophthisis. Understanding the mechanisms by which GLIS3 functions is essential for uncovering potential therapeutic targets and developing treatments for these conditions.Overall, the GLIS3 Polyclonal Antibody (CAB12166) is a reliable tool for researchers aiming to delve into the intricate roles of GLIS3 in cellular processes and disease pathogenesis. Its specificity, sensitivity, and versatility make it a valuable asset for advancing our understanding of this important transcription factor.
Product Name:
GLIS3 Rabbit Polyclonal Antibody
SKU:
CAB12166
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 606-775 of human GLIS3 (NP_689842.3).
This gene is a member of the GLI-similar zinc finger protein family and encodes a nuclear protein with five C2H2-type zinc finger domains. This protein functions as both a repressor and activator of transcription and is specifically involved in the development of pancreatic beta cells, the thyroid, eye, liver and kidney. Mutations in this gene have been associated with neonatal diabetes and congenital hypothyroidism (NDH). Alternatively spliced variants that encode different protein isoforms have been described but the full-length nature of only two have been determined.
Purification Method:
Affinity purification
Gene ID:
169792
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates, using GLIS3 Rabbit pAb (CAB12166) at 1:7000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
