The GLI2 Polyclonal Antibody (CAB6510) is a valuable tool for researchers studying the GLI2 protein, a transcription factor involved in the Hedgehog signaling pathway. This antibody, produced in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By binding to the GLI2 protein, this antibody enables researchers to accurately detect and analyze GLI2 expression in various cell types.GLI2 is a key player in the regulation of cell growth and differentiation, making it a crucial target for research in developmental biology and cancer.
Dysregulation of the Hedgehog pathway, in which GLI2 is a central component, has been implicated in a variety of diseases, including basal cell carcinoma and medulloblastoma. Understanding the role of GLI2 in these conditions is essential for the development of targeted therapies.Overall, the GLI2 Polyclonal Antibody (CAB6510) offers researchers a powerful tool for investigating the function of the GLI2 protein and its implications in disease pathology. Its high specificity and reliability make it an ideal choice for studies in cancer research and developmental biology.
Product Name:
GLI2 Rabbit Polyclonal Antibody
SKU:
CAB6510
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of mus musculus GLI2 (NP_001074594.1).
Enables DNA-binding transcription activator activity, RNA polymerase II-specific; RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; and promoter-specific chromatin binding activity. Acts upstream of or within several processes, including animal organ development; chordate embryonic development; and regulation of smoothened signaling pathway. Located in several cellular components, including axoneme; ciliary tip; and nuclear speck. Is expressed in several structures, including central nervous system; embryo mesenchyme; eye; genitourinary system; and jaw. Human ortholog(s) of this gene implicated in Culler-Jones syndrome; holoprosencephaly 9; and spina bifida. Orthologous to human GLI2 (GLI family zinc finger 2).
Purification Method:
Affinity purification
Gene ID:
14633
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GLI2 antibody (CAB6510) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
