The GLI1 Polyclonal Antibody (CAB8387) is a valuable tool for researchers studying the GLI1 protein, a key transcription factor implicated in the Hedgehog signaling pathway. This antibody, generated in rabbits, is highly specific for human samples and has been validated for use in Western blot applications. By targeting the GLI1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it ideal for studies in developmental biology, cancer research, and stem cell biology.GLI1 plays a crucial role in regulating gene expression, cell growth, and differentiation, making it a significant player in various cellular processes.
Dysregulation of the Hedgehog pathway and GLI1 activity has been implicated in a range of diseases, including cancer, developmental disorders, and degenerative conditions. Understanding the function of GLI1 is essential for uncovering new therapeutic targets and developing strategies for treating related disorders. Overall, the GLI1 Polyclonal Antibody (CAB8387) offers researchers a reliable tool for investigating the role of GLI1 in health and disease, providing valuable insights into the mechanisms underlying Hedgehog signaling and its implications for human biology.
Product Name:
GLI1 Rabbit Polyclonal Antibody
SKU:
CAB8387
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant protein of human GLI1
Tested Applications:
WBELISA
Recommended Dilution:
WB,1:500 - 1:2000
Synonyms:
GLI; PPD1; PAPA8; GLI1
Positive Sample:
Mouse testis
Conjugate:
Unconjugated
Cellular Localization:
Cytoplasm, Nucleus, Nucleus.
Calculated MW:
118kDa
Observed MW:
160kDa
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2735
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of Mouse testis, using GLI1 Rabbit pAb (CAB8387) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 180s.
