The GLI1 Polyclonal Antibody (CAB14675) is a valuable tool for researchers studying the GLI1 protein, a transcription factor implicated in various cellular processes including development, differentiation, and tumorigenesis. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By specifically binding to the GLI1 protein, it enables precise detection and analysis in a variety of cell types, making it an ideal choice for investigations in the fields of developmental biology and cancer research.GLI1 is a key regulator of the Hedgehog signaling pathway, playing a critical role in embryonic development and tissue homeostasis.
Dysregulation of GLI1 has been associated with various cancers, making it a promising target for therapeutic interventions. Understanding the function of GLI1 is essential for unraveling its role in disease progression and identifying potential treatment strategies for cancer and other related disorders. The GLI1 Polyclonal Antibody provides researchers with a reliable tool to further explore the intricacies of GLI1 signaling and its implications in both normal physiology and disease processes.
Product Name:
GLI1 Rabbit Polyclonal Antibody
SKU:
CAB14675
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 350-450 of human GLI1 (NP_001153517.1).
This gene encodes a member of the Kruppel family of zinc finger proteins. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Multiple transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2735
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.05% proclin300,50% glycerol,pH7.3.
Western blot analysis of lysates from Mouse brain, using GLI1 Rabbit pAb (CAB14675) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
