The GLE1 Polyclonal Antibody (CAB13207) is a valuable tool for researchers studying GLE1, a protein involved in mRNA export and translation regulation. This antibody, produced in rabbits, exhibits high reactivity towards human samples and is validated for use in Western blot applications. By binding specifically to the GLE1 protein, this antibody allows for accurate detection and analysis in various cell types, making it ideal for investigations in molecular biology and gene expression studies.GLE1 is a crucial component of the cellular machinery responsible for mRNA transport and processing, making it essential for proper gene expression and cellular function.
Dysregulation of GLE1 has been implicated in various diseases, including neurodegenerative disorders and certain cancers, highlighting its importance as a potential therapeutic target. Research into the role of GLE1 can provide valuable insights into these disease mechanisms and potentially lead to the development of novel treatment strategies.
Product Name:
GLE1 Rabbit Polyclonal Antibody
SKU:
CAB13207
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GLE1 (NP_001003722.1).
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2733
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of lysates from Jurkat cells, using GLE1 Rabbit pAb (CAB13207) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
