The GLE1 Polyclonal Antibody (CAB13206) is a valuable tool for researchers studying the GLE1 protein, which is involved in RNA metabolism and mRNA export from the nucleus to the cytoplasm. This antibody, produced in rabbits, exhibits high reactivity with human samples and has been validated for use in Western blot applications. By binding to the GLE1 protein, this antibody enables precise detection and analysis in a variety of cell types, making it well-suited for investigations in molecular biology and gene expression.The GLE1 protein is essential for the efficient export of mRNA, which is crucial for gene expression and cellular function.
Dysregulation of mRNA export can lead to various diseases, including cancer and neurodegenerative disorders. Research on the GLE1 protein is instrumental in understanding the mechanisms underlying these conditions and developing potential therapeutic interventions. The GLE1 Polyclonal Antibody provides researchers with a reliable tool to explore the functions and interactions of the GLE1 protein, furthering our knowledge of RNA biology and disease pathology.
Product Name:
GLE1 Rabbit Polyclonal Antibody
SKU:
CAB13206
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GLE1 (NP_001003722.1).
This gene encodes a predicted 75-kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly(A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly(A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2733
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using GLE1 Rabbit pAb (CAB13206) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 1s.
