The GLDC Polyclonal Antibody (CAB9933) is a crucial tool for studying the glycine decarboxylase (GLDC) protein involved in metabolic processes and amino acid metabolism. This antibody, produced in rabbits, exhibits high specificity and sensitivity for detecting GLDC in human samples, making it an excellent choice for Western blot applications.GLDC plays a vital role in the breakdown of glycine, an essential amino acid involved in numerous cellular processes. Dysregulation of GLDC has been associated with a variety of metabolic disorders and neurological conditions, making it a key target for research in these areas.
By using the GLDC Polyclonal Antibody, researchers can accurately measure and analyze the expression levels of GLDC in different cell types and tissues, providing valuable insights into its function and potential therapeutic implications.Whether investigating the role of GLDC in metabolic pathways, neurological disorders, or other physiological processes, the GLDC Polyclonal Antibody is a valuable tool for researchers seeking to deepen their understanding of this critical protein. Its high specificity and reliability make it a trusted asset for studies in biochemistry, molecular biology, and beyond.
Product Name:
GLDC Rabbit Polyclonal Antibody
SKU:
CAB9933
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 36-290 of human GLDC (NP_000161.2).
WB,1:500 - 1:2000 IF/ICC,1:50 - 1:100 IP,0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Synonyms:
GCE; GCSP; HYGN1; GLDC
Positive Sample:
HepG2,DU145
Conjugate:
Unconjugated
Cellular Localization:
Mitochondrion.
Calculated MW:
113kDa
Observed MW:
113kDa
Degradation of glycine is brought about by the glycine cleavage system, which is composed of four mitochondrial protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase), H protein (a lipoic acid-containing protein), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase). The protein encoded by this gene is the P protein, which binds to glycine and enables the methylamine group from glycine to be transferred to the T protein. Defects in this gene are a cause of nonketotic hyperglycinemia (NKH).
Purification Method:
Affinity purification
Gene ID:
2731
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using GLDC Rabbit pAb (CAB9933) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
