The GLA Polyclonal Antibody (CAB13987) is a valuable tool for researchers studying GLA, a key enzyme involved in lipid metabolism. This antibody, generated in rabbits, shows high reactivity with human samples and has been validated for use in Western blot applications. By targeting the GLA protein, this antibody allows for precise detection and analysis in a variety of cell types, making it an essential resource for studies in metabolism and lipid-related diseases.GLA, also known as alpha-galactosidase A, plays a crucial role in breaking down complex carbohydrates and lipids in the body.
Mutations in the GLA gene can lead to enzyme deficiency, resulting in conditions like Fabry disease, a rare genetic disorder impacting multiple organs. Research focused on understanding the function and regulation of GLA is essential for developing targeted therapies and improving outcomes for individuals affected by these lipid metabolism disorders.
Recombinant fusion protein containing a sequence corresponding to amino acids 150-429 of human Galactosidase alpha (Galactosidase alpha (GLA)) (NP_000160.1).
This gene encodes a homodimeric glycoprotein that hydrolyses the terminal alpha-galactosyl moieties from glycolipids and glycoproteins. This enzyme predominantly hydrolyzes ceramide trihexoside, and it can catalyze the hydrolysis of melibiose into galactose and glucose. A variety of mutations in this gene affect the synthesis, processing, and stability of this enzyme, which causes Fabry disease, a rare lysosomal storage disorder that results from a failure to catabolize alpha-D-galactosyl glycolipid moieties.
Purification Method:
Affinity purification
Gene ID:
2717
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of HeLa cells, using Galactosidase alpha (Galactosidase alpha (GLA)) antibody (CAB13987) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
