The GJB1 Polyclonal Antibody (CAB10112) is specifically designed for research involving the GJB1 protein, also known as connexin-32. This cell surface protein is a member of the gap junction protein family and is crucial for the communication and coordination of cells in various tissues. Raised in rabbits, this polyclonal antibody is highly reactive with human samples and has been validated for use in Western blot applications. It binds specifically to the GJB1 protein, allowing for accurate detection and analysis in a variety of cell types. The GJB1 protein is known for its role in the functioning of gap junctions, which are essential for the direct exchange of ions and small molecules between adjacent cells.
Mutations in the GJB1 gene have been associated with various diseases, including X-linked Charcot-Marie-Tooth disease, a neurological disorder affecting peripheral nerves. Research into the GJB1 protein and its related pathways is critical for understanding the mechanisms underlying these diseases and identifying potential therapeutic targets. The GJB1 Polyclonal Antibody is a valuable tool for investigators studying gap junction function, cellular communication, and related disorders.
Product Name:
GJB1 Rabbit Polyclonal Antibody
SKU:
CAB10112
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 214-283 of human GJB1 (NP_000157.1).
Sequence:
IRAC ARRA QRRS NPPS RKGS GFGH RLSP EYKQ NEIN KLLS EQDG SLKD ILRR SPGT GAGL AEKS DRCS AC
Cell junction, Cell membrane, Multi-pass membrane protein, gap junction.
Calculated MW:
32kDa
Observed MW:
32kDa/36kDa
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Purification Method:
Affinity purification
Gene ID:
2705
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GJB1 antibody (CAB10112) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
