The GJA5 Polyclonal Antibody (CAB7231) is a valuable tool for researchers studying the connexin-40 (GJA5) protein, which plays a crucial role in intercellular communication within the heart. The antibody, produced in rabbits, demonstrates high reactivity with human samples and is specifically validated for use in Western blot applications. By binding to the GJA5 protein, this antibody enables accurate detection and analysis in a variety of cell types, making it an ideal choice for studies in cardiology and cardiovascular research.
Connexin-40 is a key component of gap junction channels in the heart, facilitating the spread of electrical impulses between cardiac cells and contributing to proper heart function. Dysregulation of GJA5 has been implicated in various cardiac disorders, including arrhythmias and structural heart disease. By studying the role of GJA5 in intercellular communication, researchers can gain insights into the mechanisms underlying these conditions and potentially identify new therapeutic targets for cardiovascular diseases.
Product Name:
GJA5 Rabbit Polyclonal Antibody
SKU:
CAB7231
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 229-358 of human GJA5 (NP_859054.1).
Cell junction, Cell membrane, Multi-pass membrane protein, gap junction.
Calculated MW:
40kDa
Observed MW:
40kDa
This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene may be associated with atrial fibrillation. Alternatively spliced transcript variants encoding the same isoform have been described.
Purification Method:
Affinity purification
Gene ID:
2702
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GJA5 antibody (CAB7231) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25μg per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (AbGn00021)._Exposure time: 60s.
