The Ghrelin Polyclonal Antibody (CAB1338) is a valuable tool for researchers studying the hormone ghrelin, known for its role in regulating appetite and energy metabolism. This antibody, produced in rabbits, is highly specific for human ghrelin and has been validated for use in various experimental techniques, including Western blotting.Ghrelin is a multifunctional peptide hormone that has been linked to numerous physiological processes, including growth hormone release, food intake, and glucose metabolism. Dysregulation of ghrelin signaling has been implicated in conditions such as obesity, diabetes, and gastrointestinal disorders.
By using the Ghrelin Polyclonal Antibody, researchers can effectively detect and quantify ghrelin levels in different biological samples, allowing for a better understanding of its role in health and disease. This antibody is a valuable tool for studies in endocrinology, metabolism, and nutrition, providing insights that may lead to the development of novel therapeutic strategies targeting ghrelin signaling pathways.
Product Name:
GHRL Rabbit Polyclonal Antibody
SKU:
CAB1338
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GHRL (NP_057446.1).
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.
Purification Method:
Affinity purification
Gene ID:
51738
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GHRL antibody (CAB1338) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
