The Ghrelin Polyclonal Antibody (CAB12581) is a valuable tool for researchers studying ghrelin, a hormone that plays a significant role in regulating appetite, food intake, and energy balance. This antibody, generated in rabbits, has high reactivity with human samples and is suitable for use in Western blot applications.Ghrelin is primarily produced in the stomach and acts on the hypothalamus to stimulate hunger and promote food intake. It also has important roles in regulating metabolism, glucose homeostasis, and body weight. Dysregulation of ghrelin signaling has been implicated in conditions such as obesity, metabolic disorders, and eating disorders, making it a key target for investigation in these areas.
By utilizing the Ghrelin Polyclonal Antibody, researchers can specifically detect and analyze ghrelin protein expression in various cell types and tissues, providing valuable insights into its physiological and pathophysiological functions. This antibody is a valuable tool for studies in endocrinology, metabolism, and obesity research, offering researchers the opportunity to further understand the role of ghrelin in health and disease.
Product Name:
GHRL Rabbit Polyclonal Antibody
SKU:
CAB12581
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GHRL (NP_057446.1).
This gene encodes the ghrelin-obestatin preproprotein that is cleaved to yield two peptides, ghrelin and obestatin. Ghrelin is a powerful appetite stimulant and plays an important role in energy homeostasis. Its secretion is initiated when the stomach is empty, whereupon it binds to the growth hormone secretagogue receptor in the hypothalamus which results in the secretion of growth hormone (somatotropin). Ghrelin is thought to regulate multiple activities, including hunger, reward perception via the mesolimbic pathway, gastric acid secretion, gastrointestinal motility, and pancreatic glucose-stimulated insulin secretion. It was initially proposed that obestatin plays an opposing role to ghrelin by promoting satiety and thus decreasing food intake, but this action is still debated. Recent reports suggest multiple metabolic roles for obestatin, including regulating adipocyte function and glucose metabolism. Alternative splicing results in multiple transcript variants. In addition, antisense transcripts for this gene have been identified and may potentially regulate ghrelin-obestatin preproprotein expression.
Purification Method:
Affinity purification
Gene ID:
51738
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from rat liver, using GHRL Rabbit pAb (CAB12581) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (AbGn00021).Exposure time: 90s.
