The GGA3 Polyclonal Antibody (PAC052834) is a valuable tool for researchers studying GGA3, a crucial component of the cellular trafficking machinery. This antibody, raised in rabbits, exhibits high reactivity with human samples and is validated for use in Western blot applications. By specifically binding to the GGA3 protein, this antibody enables researchers to detect and analyze GGA3 expression in various cell types, making it ideal for studies in cell biology and disease research.GGA3 is involved in the sorting and trafficking of proteins within cells, playing a key role in maintaining cellular homeostasis and function. Dysregulation of GGA3 has been implicated in various diseases, including neurodegenerative disorders and cancer.
Understanding the function of GGA3 is essential for unraveling the molecular mechanisms underlying these conditions and developing targeted therapies.By using the GGA3 Polyclonal Antibody, researchers can gain valuable insights into the roles and regulation of GGA3 in cellular processes, paving the way for advancements in drug discovery and personalized medicine. Whether studying protein trafficking pathways or investigating disease mechanisms, this antibody is a valuable tool for elucidating the complex functions of GGA3 in health and disease.
Antibody Name:
GGA3 Antibody (PACO52834)
Antibody SKU:
PACO52834
Size:
50ug
Host Species:
Rabbit
Tested Applications:
ELISA, IHC
Recommended Dilutions:
ELISA:1:2000-1:10000, IHC:1:20-1:200
Species Reactivity:
Human
Immunogen:
Recombinant Human ADP-ribosylation factor-binding protein GGA3 protein (342-503AA)
Immunohistochemistry of paraffin-embedded human pancreatic cancer using PACO52834 at dilution of 1:100.
Background:
Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acid, c cluster-dileucine (AC-LL) motif.
Synonyms:
ADP-ribosylation factor-binding protein GGA3 (Golgi-localized, γ ear-containing, ARF-binding protein 3), GGA3, KIAA0154
UniProt Protein Function:
GGA3: Plays a role in protein sorting and trafficking between the trans-Golgi network (TGN) and endosomes. Mediates the ARF- dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Monomer. Interacts with SORT1, SORL1, LRP3, GGA binding partner (GGABP) and P200. Interacts with GGA1 and GGA2. Binds to clathrin and activated ARFs. Binds RABEP1 and RABGEF1. Interacts with the membrane proteins M6PR/CD-MPR, IGF2R/CI-MPR and BACE1 and the accessory proteins SYNRG, EPN4, NECAP1, NECAP2 and AFTPH/aftiphilin. Interacts with TSG101 and UBC. Ubiquitously expressed. Belongs to the GGA protein family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Vesicle; Adaptor/scaffoldChromosomal Location of Human Ortholog: 17q25.1Cellular Component: clathrin adaptor complex; endosome membrane; trans-Golgi networkMolecular Function: ADP-ribosylation factor binding; protein bindingBiological Process: vesicle-mediated transport; intracellular protein transport; positive regulation of protein catabolic process
UniProt Protein Details:
NCBI Summary:
This gene encodes a member of the Golgi-localized, gamma adaptin ear-containing, ARF-binding (GGA) family. This family includes ubiquitous coat proteins that regulate the trafficking of proteins between the trans-Golgi network and the lysosome. These proteins share an amino-terminal VHS domain which mediates sorting of the mannose 6-phosphate receptors at the trans-Golgi network. They also contain a carboxy-terminal region with homology to the ear domain of gamma-adaptins. Multiple alternatively spliced transcript variants have been identified in this gene. [provided by RefSeq, Feb 2010]
