The GFP Monoclonal Antibody (PACO13759) is a specialized tool for researchers working with green fluorescent protein (GFP). This antibody, produced through a specific process, demonstrates high affinity and specificity for GFP, making it an excellent choice for various experimental applications.GFP is a widely used and versatile tool in molecular and cell biology, commonly utilized as a marker for gene expression, protein localization, and cell tracking. The GFP Monoclonal Antibody allows for the accurate detection and visualization of GFP-tagged proteins in a variety of research settings, including immunofluorescence, flow cytometry, and fluorescent microscopy.
With its exceptional sensitivity and selectivity, the GFP Monoclonal Antibody offers researchers a reliable and effective means of studying GFP-expressing cells and proteins. Whether investigating gene expression dynamics, cellular processes, or protein interactions, this antibody is an essential resource for advancing scientific understanding in fields such as genetics, molecular biology, and biotechnology.
Antibody Name:
GFP Antibody (PACO13759)
Antibody SKU:
PACO13759
Size:
50ul
Host Species:
Rabbit
Tested Applications:
ELISA, WB
Recommended Dilutions:
ELISA:1:2000-1:10000, WB:1:1000-1:10000
Species Reactivity:
Human
Immunogen:
Fusion protein of Aequorea Victoria GFP
Form:
Liquid
Storage Buffer:
-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:
Antigen affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Conjugate:
Non-conjugated
Gel: 8%SDS-PAGE, Lysate: 1,5,10,20 μg, Lane: GFP transfected 293T cells, Primary antibody: PACO13759(GFP Antibody) at dilution 1/800, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds.
Background:
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). Green Fluorescent Protein (GFP) has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells.
Synonyms:
Green fluorescent protein
UniProt Protein Function:
Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.
