The GenieFluor Violet 450 Anti-Mouse LY6A/E (Sca-1) Antibody (D7) is a highly specific antibody designed for research involving LY6A/E, a cell surface marker often used to identify stem and progenitor cells in mice. This antibody, compatible with flow cytometry and immunofluorescence applications, is produced with high purity and specificity for accurate and reliable results in your experiments. LY6A/E, also known as Sca-1, is a key marker in the field of stem cell biology and is crucial for identifying and studying different cell populations within the mouse model system.
By using this antibody, researchers can effectively target and analyze LY6A/E-positive cells in various tissues, offering valuable insights into stem cell populations and their roles in development, regeneration, and disease processes. Whether investigating stem cell biology, tissue regeneration, or immune responses, the GenieFluor Violet 450 Anti-Mouse LY6A/E (Sca-1) Antibody (D7) is an essential tool for researchers looking to advance their understanding of stem cell dynamics and function in mouse models.
Genie Fluor Violet 450 Rat IgG2a, κ Isotype Control[2A3]
Form:
Liquid
Conjugation:
Genie FluorViolet 450
Recommended Use:
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P05533
Gene ID:
110454
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses.