The GenieFluorâ„¢ 488 Anti-Human CD34 Antibody (4H11) is a vital tool for researchers in the field of immunology and stem cell biology. This monoclonal antibody, specifically targeting the CD34 antigen present on hematopoietic stem cells and endothelial cells, is a key reagent for identifying and isolating these important cell populations in both basic research and clinical applications.The 4H11 antibody, meticulously developed and validated for its specificity and sensitivity, offers robust performance in various experimental techniques such as flow cytometry, immunofluorescence, and immunohistochemistry. By selectively binding to the CD34 protein, this antibody facilitates the precise visualization and characterization of CD34-expressing cells, aiding in the identification of stem cells, progenitor cells, and vascular endothelial cells.
Researchers studying hematopoiesis, angiogenesis, cancer biology, and regenerative medicine will benefit greatly from the high quality and reliability of the GenieFluorâ„¢ 488 Anti-Human CD34 Antibody (4H11). With its ability to accurately detect and quantify CD34-positive cells, this antibody opens up possibilities for groundbreaking discoveries and advancements in various areas of biomedical research.
Each lot of this antibody is quality control tested by flow cytometric analysis. The amount of the reagent is suggested to be used 5 µL of antibody per test (million cells in 100 µL staining volume or per 100 µL of whole blood). Please check your vial before the experiment. Since applications vary, the appropriate dilutions must be determined for individual use.
Spectrum:
Swissprot:
P28906
Gene ID:
947
Storage Buffer:
Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Stability & Storage:
Keep as concentrated solution. Store at 2~8°C and protected from prolonged exposure to light. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Background:
CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.