The General Vitamin B9 (VB9) ELISA Kit from Assay Genie is a cutting-edge tool designed for the precise quantification of Vitamin B9 levels in various biological samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it an invaluable asset for researchers in a wide range of fields.Vitamin B9, also known as folic acid, is an essential nutrient crucial for numerous biological processes including DNA synthesis, cell growth, and repair. Inadequate levels of Vitamin B9 have been linked to various health issues such as anemia, birth defects, and cardiovascular diseases.
This ELISA kit enables researchers to easily measure Vitamin B9 levels, leading to a better understanding of its role in health and disease.Whether you are studying Vitamin B9 deficiency, exploring its impact on various health conditions, or developing interventions to optimize Vitamin B9 levels, the General Vitamin B9 (VB9) ELISA Kit from Assay Genie is the reliable and efficient solution for your research needs. Order yours today and unlock the potential of Vitamin B9 research.
Product Name:
General Vitamin B9 (VB9) ELISA Kit
Product Code:
UNDL00081
Size:
96 Assays
Target:
VB9
Synonyms:
FA, Folic Acid, Folacin, Folate, V, Itamin M, Vitamin M, Vitamin Bc, Vitamin B9, Pteroyl-L-Glutamic Acid, Pteroyl-L-Glutamate
Detection Method:
Competition
Range:
3.90-250ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
