The General Vitamin B1 (VB1) ELISA Kit is a reliable tool for the precise measurement of Vitamin B1 levels in various biological samples including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and reproducible results, making it an essential component for research studies in the fields of nutrition, metabolism, and health.Vitamin B1, also known as Thiamine, is an essential nutrient that plays a crucial role in energy metabolism, nerve function, and maintaining a healthy cardiovascular system. Deficiency in Vitamin B1 can lead to a range of health issues, including beriberi and Wernicke-Korsakoff syndrome.
By accurately measuring Vitamin B1 levels, researchers can gain valuable insights into the impact of this essential nutrient on various physiological processes and diseases.Whether investigating the role of Vitamin B1 in nutritional deficiencies, neurological disorders, or cardiovascular health, the General Vitamin B1 (VB1) ELISA Kit provides a reliable and efficient solution for quantitative analysis. Trust in this kit to support your research endeavors and advance understanding of the importance of Vitamin B1 in human health and disease.
Product Name:
General Vitamin B1 (VB1) ELISA Kit
Product Code:
UNDL00078
Size:
96 Assays
Target:
VB1
Synonyms:
Thiamine, Thiamin, Aneurine
Detection Method:
Competition
Range:
7.81-500ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
