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Lipopolysaccharides (LPS) ELISA Kit

SKU:
UNDL00050
Product Type:
ELISA Kit
Sensitivity:
1.24ng/mL
Range:
3.12-200ng/mL
ELISA Type:
Competitive
Synonyms:
LOS,Lipoglycans,Lipooligosaccharide,Lipo-Oligosaccharide,Endotoxin
Reactivity:
Universal
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

General Lipopolysaccharides (LPS) ELISA Kit

The General Lipopolysaccharides (LPS) ELISA Kit is a reliable and accurate tool for detecting lipopolysaccharides in various samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.Lipopolysaccharides are essential components of the outer membrane of Gram-negative bacteria, playing crucial roles in cell structure and function. Detection of LPS is important in the study of bacterial infections, immune responses, and inflammatory processes, making this kit a valuable asset for researchers in microbiology, immunology, and infectious disease studies.

With its ease of use and proven performance, the General Lipopolysaccharides (LPS) ELISA Kit is a versatile tool for investigating the role of LPS in biological processes and diseases, providing valuable insights for potential therapeutic interventions.

Product Name:General Lipopolysaccharides (LPS) ELISA Kit
Product Code:UNDL00050
Size:96 Assays
Target:LPS
Synonyms:LOS, Lipoglycans, Lipooligosaccharide, Lipo-Oligosaccharide, Endotoxin
Detection Method:Competition
Range:3.12-200ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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