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General Homovanillic Acid (HVA) ELISA Kit

SKU:
UNDL00046
Product Type:
ELISA Kit
Sensitivity:
0.337ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Competitive
Synonyms:
HOC6H3(OCH3)CH2COOH,3-Methoxy-4-hydroxyphenyl Acetic Acid,4-Hydroxy-3-Methoxy-Benzeneacetic Acid,4-Hydroxy-3-Methoxyphenylacetic Acid
Reactivity:
Universal
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

system_update_altDatasheet

General Homovanillic Acid (HVA) ELISA Kit

The General Homovanillic Acid (HVA) ELISA Kit from Assay Genie is a cutting-edge tool for accurate detection of HVA levels in biological samples. This kit offers high sensitivity and specificity, allowing researchers to obtain reliable and reproducible results for a variety of research applications.Homovanillic Acid (HVA) is a metabolite of dopamine and a key player in the central nervous system. Its levels are associated with various neurological and psychiatric disorders, making it a valuable biomarker for studying these conditions and potential therapeutic interventions.

With the General Homovanillic Acid (HVA) ELISA Kit, researchers can delve deeper into the role of HVA in these disorders and explore new avenues for diagnosis and treatment. Don't miss out on this powerful tool for advancing your research in neuroscience and beyond. Order your kit today from Assay Genie.

Product Name:General Homovanillic Acid (HVA) ELISA Kit
Product Code:UNDL00046
Size:96 Assays
Target:HVA
Synonyms:HOC6H3(OCH3)CH2COOH, 3-Methoxy-4-hydroxyphenyl Acetic Acid, 4-Hydroxy-3-Methoxy-Benzeneacetic Acid, 4-Hydroxy-3-Methoxyphenylacetic Acid
Detection Method:Competition
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.Aspirate and wash 3 times
4.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.Aspirate and wash 5 times
6.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.Add 50µL Stop Solution. Read at 450 nm immediately.

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