The General Estrone Sulfate (E1S) ELISA Kit from Assay Genie is a comprehensive tool for accurately measuring levels of estrone sulfate in various biological samples. This kit offers high sensitivity and specificity, guaranteeing precise and consistent results for research applications.Estrone sulfate is a key estrogen metabolite known for its role in hormone-regulated processes such as menstrual cycling and pregnancy. Elevated levels of E1S have been associated with conditions like breast cancer and osteoporosis, making it a valuable biomarker for studying these diseases and developing targeted treatments.
With its user-friendly protocols and reliable performance, the General Estrone Sulfate ELISA Kit is an essential resource for researchers and clinicians looking to investigate the impact of estrone sulfate on human health. Visit the Assay Genie website to learn more about this innovative product.
Product Name:
General Estrone Sulfate (E1S) ELISA Kit
Product Code:
UNDL00031
Size:
96 Assays
Target:
E1S
Detection Method:
Competition
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-102
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
