The General Estradiol E2 ELISA Kit offered by Assay Genie is a cutting-edge tool for the accurate measurement of estradiol levels in various biological samples, including serum, plasma, and tissue culture supernatants. This kit boasts exceptional sensitivity and specificity, ensuring precise and consistent results, making it an excellent choice for a wide array of research endeavors. Estradiol, a type of estrogen, is a critical hormone involved in numerous physiological processes, including reproductive health and bone density regulation.
Dysregulation of estradiol levels has been linked to various disorders, such as hormonal imbalances, infertility, and menopausal symptoms, highlighting its importance as a biomarker for medical research and diagnostic purposes. The General Estradiol E2 ELISA Kit from Assay Genie is a valuable asset for researchers and healthcare professionals seeking to explore the roles of estradiol in health and disease, paving the way for innovative treatments and interventions in the field of endocrinology and beyond.
Product Name:
General Estradiol (E2) ELISA Kit
Product Code:
UNDL00032
Size:
96 Assays
Target:
E2
Synonyms:
17B-Estradiol, Oestradiol, Beta-Estradiol
Detection Method:
Competition
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
