The General Carnosine CAR ELISA Kit is a reliable and accurate tool for detecting carnosine levels in a variety of biological samples, including serum, plasma, and cell culture supernatants. This kit is designed with high sensitivity and specificity to ensure precise and reproducible results, making it perfect for a wide range of research applications.Carnosine is a dipeptide molecule that plays a key role in cellular functions, antioxidant activities, and anti-aging processes. It has been shown to have beneficial effects on muscle function, brain health, and overall well-being.
By measuring carnosine levels, researchers can gain valuable insights into its potential therapeutic benefits for various health conditions.Whether studying the effects of carnosine supplementation, assessing its role in disease pathology, or exploring its anti-aging properties, the General Carnosine CAR ELISA Kit provides a powerful tool for advancing scientific research and uncovering new discoveries in the field of biochemistry and molecular biology.
Product Name:
General Carnosine (CAR) ELISA Kit
Product Code:
UNDL00018
Size:
96 Assays
Target:
CAR
Synonyms:
?-Alanyl-L-Histidine
Detection Method:
Competition
Range:
15.625-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well. And then add 50µL prepared Detection Reagent A immediately. Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
