The General Adenosine Triphosphate (ATP) ELISA Kit is a powerful tool for the accurate measurement of ATP levels in various biological samples including cell lysates, tissue homogenates, and serum. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a wide range of research applications.ATP is a vital molecule involved in cellular energy metabolism, serving as a primary source of energy for various cellular processes. Changes in ATP levels can impact cellular functions and signaling pathways, making it a key indicator of cellular health and function.
Understanding ATP levels can provide valuable insights into cellular metabolism, oxidative stress, and mitochondrial function, making this kit essential for research in areas such as metabolism, aging, and neurodegenerative diseases.Overall, the General Adenosine Triphosphate (ATP) ELISA Kit is an invaluable tool for researchers seeking to accurately measure ATP levels in biological samples and investigate the role of ATP in various physiological and pathological processes.
Product Name:
General Adenosine Triphosphate (ATP) ELISA Kit
Product Code:
UNDL00015
Size:
96 Assays
Target:
ATP
Synonyms:
Adenosine-5'-Triphosphate
Detection Method:
Competition
Range:
15.6-1,000ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-99
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. Note:To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity, and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 50µL standard or sample to each well.    And then add 50µL prepared Detection Reagent A immediately.    Shake and mix. Incubate 1 hour at 37°C
3.
Aspirate and wash 3 times
4.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
5.
Aspirate and wash 5 times
6.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
7.
Add 50µL Stop Solution. Read at 450 nm immediately.
