Anti-GMNN Antibody (CAB5316)

Antibody Name:	Anti-GMNN Antibody
Antibody SKU:	CAB5316
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, 22Rv1, HL-60, MCF7, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-209 of human GMNN (NP_056979.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNPS MKQK QEEI KENI KNSS VPRR TLKM IQPS ASGS LVGR ENEL SAGL SKRK HRND HLTS TTSS PGVI VPES SENK NLGG VTQE SFDL MIKE NPSS QYWK EVAE KRRK ALYE ALKE NEKL HKEI EQKD NEIA RLKK ENKE LAEV AEHV QYMA ELIE RLNG EPLD NFES LDNQ EFDS EEET VEDS LVED SEIG TCAE GTVS SSTD AKPC I
Gene ID:	51053
Uniprot:	O75496
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	23kDa
Observed MW:	24kDa

Synonyms:

GMNN, Gem, MGORS6, geminin

Background:

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16.

UniProt Protein Function:	Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family.
UniProt Protein Details:	Protein type:Cell cycle regulation; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6p22.3 Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus Molecular Function:protein binding; histone deacetylase binding; transcription corepressor activity Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle
NCBI Summary:	This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]
UniProt Code:	O75496
NCBI GenInfo Identifier:	20454908
NCBI Gene ID:	51053
NCBI Accession:	O75496.1
UniProt Secondary Accession:	O75496,Q9H1Z1, B3KMM8,
UniProt Related Accession:	O75496
Molecular Weight:	209
NCBI Full Name:	Geminin
NCBI Synonym Full Names:	geminin, DNA replication inhibitor
NCBI Official Symbol:	GMNN
NCBI Official Synonym Symbols:	Gem
NCBI Protein Information:	geminin
UniProt Protein Name:	Geminin
Protein Family:	Geminin
UniProt Gene Name:	GMNN
UniProt Entry Name:	GEMI_HUMAN

	Western blot analysis of extracts of various cell lines, using GMNN antibody (CAB5316) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using GMNN Rabbit pAb (CAB5316) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human colon carcinoma using GMNN Rabbit pAb (CAB5316) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse testis using GMNN Rabbit pAb (CAB5316) at dilution of 1:100 (40x lens).

Antibodies

GMNN Antibody (PACO09502)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)