The GCLM Polyclonal Antibody (CAB13989) is a valuable tool for researchers studying glutamate-cysteine ligase modifier subunit (GCLM) protein in various biological contexts. This antibody, produced in rabbits, is highly specific and reactive with human samples, making it ideal for use in Western blot applications. By binding to the GCLM protein, this antibody allows for reliable detection and analysis of GCLM expression levels in different cell types.GCLM is an important regulatory enzyme involved in the synthesis of glutathione, a crucial antioxidant molecule that plays a key role in protecting cells from oxidative damage. Dysregulation of GCLM has been linked to various diseases, including cancer, neurodegenerative disorders, and inflammatory conditions.
By studying the expression and activity of GCLM using this antibody, researchers can gain insight into the role of glutathione metabolism in these diseases and potentially identify new therapeutic targets.Overall, the GCLM Polyclonal Antibody (CAB13989) is a reliable tool for investigating the functions of the GCLM protein and its implications in health and disease. Its high specificity and sensitivity make it a valuable asset for research in fields such as biochemistry, cell biology, and molecular medicine.
Product Name:
GCLM Rabbit Polyclonal Antibody
SKU:
CAB13989
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
Purification Method:
Affinity purification
Gene ID:
2730
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of various cell lines, using GCLM antibody (CAB13989) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).
