The GCLC Rabbit Monoclonal Antibody (CAB4499) is a powerful tool for researchers studying the role of the protein GCLC in cellular function. GCLC is a key enzyme involved in the synthesis of glutathione, an essential antioxidant that plays a critical role in protecting cells from oxidative stress and maintaining cellular health.This high-quality antibody, developed using rabbit monoclonal technology, offers exceptional sensitivity and specificity for detecting GCLC in a variety of samples, including human tissues and cell lines. It has been validated for use in Western blotting, immunohistochemistry, and immunofluorescence applications, providing researchers with multiple options for analyzing GCLC expression and localization.
Studies have shown that dysregulation of GCLC expression is associated with a variety of diseases, including cancer, neurodegenerative disorders, and cardiovascular disease. By using the GCLC Rabbit Monoclonal Antibody (CAB4499), researchers can gain valuable insights into the role of GCLC in these conditions and potentially identify new therapeutic targets for intervention.Overall, this antibody is a valuable resource for investigating the function of GCLC in cellular pathways and disease processes, making it an essential tool for researchers in the fields of biochemistry, molecular biology, and medicine.
Product Name:
GCLC Rabbit Monoclonal Antibody
SKU:
CAB4499
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Rat
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 538-637 of human GCLC (P48506).
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.
Purification Method:
Affinity purification
Gene ID:
2729
Clone Number:
ARC1065
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat kidney, using GCLC Rabbit mAb (CAB4499) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.
