The GCKR Polyclonal Antibody (CAB5678) is specifically designed for researchers studying GCKR, a key protein involved in glucose and lipid metabolism. This antibody, generated in rabbits, exhibits high reactivity towards human samples and has been validated for use in Western blot applications. By binding to the GCKR protein, it enables accurate detection and analysis in a variety of cell types, making it a valuable tool for studies in metabolic diseases and diabetes research.
GCKR, or glucokinase regulatory protein, is essential for regulating glucose metabolism by modulating the activity of glucokinase, a key enzyme in glucose utilization. Dysregulation of GCKR has been linked to metabolic disorders such as diabetes and hyperlipidemia, making it a target of interest for therapeutic interventions. Understanding the function and regulation of GCKR is crucial for developing targeted treatments for these metabolic conditions.
Product Name:
GCKR Rabbit Polyclonal Antibody
SKU:
CAB5678
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human GCKR (NP_001477.2).
This gene encodes a protein belonging to the GCKR subfamily of the SIS (Sugar ISomerase) family of proteins. The gene product is a regulatory protein that inhibits glucokinase in liver and pancreatic islet cells by binding non-covalently to form an inactive complex with the enzyme. This gene is considered a susceptibility gene candidate for a form of maturity-onset diabetes of the young (MODY).
Purification Method:
Affinity purification
Gene ID:
2646
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of lysates from Rat liver using GCKR Rabbit pAb (CAB5678) at 1:1000 dilution incubated overnight at 4°C.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)(CABS014) at 1:10000 dilution.Lysates/proteins: 25 μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020)Exposure time: 90 s.
