The GCK Polyclonal Antibody (CAB15059) is a crucial tool for researchers studying the glucokinase enzyme, which plays a key role in glucose metabolism and insulin secretion. This antibody, produced in rabbits, is highly specific to human samples and is validated for use in Western blot applications. By binding to the GCK protein, this antibody allows for the detection and analysis of GCK levels in various cell types, making it an invaluable tool for studies in diabetes research and metabolic disorders.Glucokinase is a critical enzyme that acts as a glucose sensor in pancreatic beta cells, helping to regulate blood sugar levels by initiating insulin release in response to elevated glucose levels.
Dysregulation of GCK activity is associated with disorders such as type 2 diabetes and hyperinsulinemia. Understanding the function and regulation of GCK is essential for developing targeted therapies for these conditions, making the GCK Polyclonal Antibody a valuable asset for researchers investigating glucose metabolism and insulin signaling pathways.
Product Name:
Glucokinase (GCK) Rabbit Polyclonal Antibody
SKU:
CAB15059
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Glucokinase (Glucokinase (GCK)) (NP_000153.1).
This gene encodes a member of the hexokinase family of proteins. Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. The use of multiple promoters and alternative splicing of this gene result in distinct protein isoforms that exhibit tissue-specific expression in the pancreas and liver. In the pancreas, this enzyme plays a role in glucose-stimulated insulin secretion, while in the liver, this enzyme is important in glucose uptake and conversion to glycogen. Mutations in this gene that alter enzyme activity have been associated with multiple types of diabetes and hyperinsulinemic hypoglycemia.
Purification Method:
Affinity purification
Gene ID:
2645
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Western blot analysis of various lysates using Glucokinase (GCK) Rabbit pAb (CAB15059) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.
