The GCDH Polyclonal Antibody (CAB9057) is a valuable tool for researchers studying GCDH, an enzyme involved in the metabolism of the amino acid lysine. This antibody, generated in rabbits, is highly specific for human samples and is suitable for use in Western blot applications. By binding to the GCDH protein, this antibody enables the detection and analysis of GCDH in various cell types, making it ideal for studies in metabolism, genetic disorders, and metabolic diseases.GCDH deficiency is a rare genetic disorder that can lead to serious health complications, including metabolic crises and neurological symptoms.
Research into GCDH and its role in lysine metabolism is essential for understanding and developing treatments for this disorder. The GCDH Polyclonal Antibody provides researchers with a powerful tool for investigating the expression and function of GCDH, leading to advances in diagnosis and potential therapies for GCDH deficiency and related conditions.
Product Name:
GCDH Rabbit Polyclonal Antibody
SKU:
CAB9057
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse,Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 149-438 of human GCDH (NP_000150.1).
The protein encoded by this gene belongs to the acyl-CoA dehydrogenase family. It catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. The enzyme exists in the mitochondrial matrix as a homotetramer of 45-kD subunits. Mutations in this gene result in the metabolic disorder glutaric aciduria type 1, which is also known as glutaric acidemia type I. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 12.
Purification Method:
Affinity purification
Gene ID:
2639
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of various lysates using GCDH Rabbit pAb (CAB9057) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 30s.
