The GBF1 Polyclonal Antibody (CAB8468) is a vital tool for researchers studying the Golgi-specific brefeldin A-resistant guanine nucleotide exchange factor 1 (GBF1) protein. This antibody, generated in rabbits, exhibits high specificity and sensitivity towards GBF1 in human samples, making it ideal for a range of research applications, including Western blotting.GBF1 plays a pivotal role in vesicular trafficking and membrane fusion processes within the Golgi apparatus, making it a key player in cellular transport and protein sorting mechanisms. Dysregulation of GBF1 has been implicated in various diseases, including neurodegenerative disorders, viral infections, and cancer.
Therefore, understanding the function and regulation of GBF1 is crucial for unraveling its role in disease pathogenesis and identifying potential therapeutic targets.By utilizing the GBF1 Polyclonal Antibody, researchers can accurately detect and analyze GBF1 expression levels in different cell types and experimental conditions. This antibody serves as a valuable tool for investigating the mechanisms underlying Golgi-related processes and exploring the potential therapeutic avenues associated with targeting GBF1 in disease settings.
Product Name:
GBF1 Rabbit Polyclonal Antibody
SKU:
CAB8468
Size:
20uL, 100uL
Isotype:
IgG
Host Species:
Rabbit
Reactivity:
Human,Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-85 of human GBF1 (NP_004184.1).
This gene encodes a member of the Sec7 domain family. The encoded protein is a guanine nucleotide exchange factor that regulates the recruitment of proteins to membranes by mediating GDP to GTP exchange. The encoded protein is localized to the Golgi apparatus and plays a role in vesicular trafficking by activating ADP ribosylation factor 1. The encoded protein has also been identified as an important host factor for viral replication. Multiple transcript variants have been observed for this gene.
Purification Method:
Affinity purification
Gene ID:
8729
Storage Buffer:
Store at -20℃. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Western blot analysis of extracts of mouse heart, using GBF1 antibody (CAB8468) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.
